论文部分内容阅读
目的:探讨不同低氧训练模式对机体骨骼肌血红素合酶(HO-1)mRNA表达的影响。方法:选用6周龄SD雄性大鼠120只,经3周适应性训练和力竭实验筛选出90只,随机分成9组:常氧安静对照组、持续低氧安静组、间歇低氧安静组、低住低练组、高住高练组、高住低练组、低住高练组、高住高练后复氧训练组、高住低练后复氧训练组。采用常压低氧舱以13.6%的氧浓度(相当于海拔3500m的氧浓度)进行低氧训练,根据血乳酸-速度曲线确定大鼠常氧训练的强度为35m/min,低氧训练的强度为30m/min。低氧训练持续时间为6周,每周训练5天。第6周末最后一次运动后休息48h后处死、取材。采用实时荧光定量PCR技术测试大鼠骨骼肌HO-1mRNA表达。结果:与常氧安静对照组相比,低住低练组大鼠骨骼肌HO-1mRNA表达显著升高(P<0.05),高住高练组、低住高练组非常显著升高(P<0.01);高住低练组与低住低练组比较显著降低(P<0.05);高住高练后复氧训练组大鼠骨骼肌HO-1mRNA表达与高住高练组相比显著降低(P<0.01),基本回到常氧安静对照组水平。结论:高住高练和低住高练可提骨骼肌HO-1mRNA表达。
Objective: To investigate the effect of different hypoxic training modes on the expression of HO-1 mRNA in the skeletal muscle of the human body. Methods: One hundred and twenty male Sprague-Dawley rats of 6 weeks old were selected. Ninety healthy SD rats were randomly divided into 9 groups: normoxia group, continuous hypoxic-quiet group, intermittent hypoxic-quiet group , Low living low training group, living high training high training group, high living low training group, living low training group, high living high rehabilitative training group, high living low rehabilitative training group. Hypoxic training was performed with atmospheric oxygen chamber at oxygen concentration of 13.6% (corresponding to an oxygen concentration of 3500m above sea level). The intensity of normoxic training was 35m / min, the intensity of hypoxic training For 30m / min. Hypoxic training duration of 6 weeks, training 5 days a week. 6 weeks after the last exercise 48h resting, drawn. Real-time fluorescence quantitative PCR was used to detect the HO-1mRNA expression in skeletal muscle of rats. Results: Compared with normoxic group, HO-1mRNA expression in skeletal muscle of low-abortion training group was significantly increased (P <0.05) <0.01). The HO-I training group was significantly lower than that in the HO-CTS training group (P <0.05). The HO-1mRNA expression in the rehabilitative training group was significantly higher than that in the Takayasu Training group (P <0.01), basically returned to the level of normoxia quiet control group. CONCLUSION: HO-1 mRNA expression in skeletal muscle can be raised by high-living-high exercise and low-abortion training.