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目的:探讨在运动心脏的发生过程中,右心室肌蛋白质组的差异表达,筛选对研究运动心脏重塑机制有重要意义的右心室蛋白质。方法:18只雄性SD大鼠按运动能力和体重随机配伍分为对照组和运动组,每组9只。运动组进行12周中等强度有氧运动(70～80%VO2max),后与对照组同时称取体重、麻醉处死,称取心脏重量,提取右心室肌的全蛋白,对样品蛋白用双向凝胶电泳技术分离、后固定染色。运用Bio-rad PD quest图像分析软件对双向凝胶电泳图谱进行分析,选取差异蛋白进行质谱鉴定。结果:经过12周运动后,运动组心脏出现明显形态学变化。此次实验获得了重复性和分辨率较好的双向凝胶电泳图谱,与对照组相比,通过软件分析,上调到10倍以上或下调至1/10及以下的差异点31个,其中7个上调,24个下调。这些点最多位于分子量50～70kDa和10～20kDa、等电点7.0～9.0范围内。质谱分析并鉴定了其中2个蛋白点,包括丙酮酸脱氢酶E1α1(运动12周后下调)和一个未知蛋白。结论:12周运动后大鼠右室肌蛋白质组发生了明显变化。能量代谢酶的变化提示中等强度运动对心脏产生的重塑作用可能主要引起右心室肌能量代谢水平的变化。
Objective: To explore the differential expression of proteomics of right ventricular muscle in the process of exercise heart, and to screen the right ventricular protein that is important for studying the mechanism of exercise heart remodeling. Methods: Eighteen male Sprague-Dawley rats were randomly divided into control group and exercise group according to their exercise capacity and body weight, with 9 rats in each group. Exercise group for 12 weeks of moderate-intensity aerobic exercise (70 ~ 80% VO2max), after weighing the same time with the control group, anesthetized sacrifice, weighed the heart weight, extract the whole protein of right ventricular muscle, the sample protein using two-way gel Electrophoresis separation, fixed after staining. The Bio-rad PD quest image analysis software was used to analyze the two-dimensional gel electrophoresis pattern, and the differential proteins were selected for mass spectrometry identification. Results: After 12 weeks of exercise, the exercise group showed obvious morphological changes in the heart. Compared with the control group, the two-way gel electrophoresis pattern with better repeatability and resolution was obtained in this experiment, which was up-regulated by software analysis to 31 times or down to 1/10 and below, of which 7 A rise, 24 down. Most of these points are located in the molecular weight of 50 ~ 70kDa and 10 ~ 20kDa, isoelectric point 7.0 ~ 9.0 range. Mass spectrometry analysis and identification of two of the protein spots, including pyruvate dehydrogenase E1α1 (12 weeks after exercise down) and an unknown protein. Conclusion: After 12 weeks’ exercise, the proteomics of right ventricular muscle of rats changed obviously. Changes in energy metabolism enzymes suggest that the remodeling effect of moderate-intensity exercise on the heart may mainly induce changes in the energy metabolism of right ventricular muscle.