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以4个抗旱性不同的水稻品种为材料,研究水分胁迫对花药培养愈伤组织诱导、苗期生长量、成熟期单株产量,以及苗期生理生化指标的影响。结果表明,水分胁迫下,‘沪旱2B’和‘沪旱3号’的花药愈伤组织诱导率、苗期生长量和单株籽粒产量的受抑程度低于‘日本晴’和‘大华香粳’;苗期叶中的脯氨酸(Pro)含量以及过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的受抑程度也是‘沪旱2B’和‘沪旱3号’低于‘日本晴’和‘大华香粳’。表明供试材料离体花药对水分胁迫的培养反应与植株水平的抗旱性存在相关性。
Four drought-resistant rice cultivars were used as materials to study the effects of water stress on callus induction, seedling growth, yield per plant, and physiological and biochemical indexes at seedling stage. The results showed that under drought stress, the anther callus induction rate, seedling growth rate and grain yield per plant were lower than those of ’Nipponbun’ and ’Da Hua Hong Japonica ’; the proline (Pro) content in seedling leaves and the degree of inhibition of peroxidase (POD) and superoxide dismutase (SOD) activity were also “Shanghai drought 2B” and “Shanghai drought 3” Lower than ’Nipponbare’ and ’Dahua Xiangjing’. The results showed that there was a correlation between the culture response of the anther to water stress and the drought resistance at the plant level.