Chitosan(CS)nanofibers containing silver nanoparticles(AgNPs)were prepared by in-situ reducing method.A water soluble carboxymethyl chitosan(CMCT)was applied for the preparation of AgNPs.The impact factor such as the concentration of CMCT,silver nitrate(AgNO_3)content,temperature and the heating time during the preparation of AgNPs were studied.The result showed that the proper value of the concentration of CMCT,AgNO_3content,temperature and the heating time were set as0.1%,20μL AgNO_3(1.7 mol/L),90°and 3 h,separately and the maximum concentration of AgNPs could be acquired.To solve the spinnability of chitosan nanofiber,a super high molecular weight polyethylene oxide(PEO)was introduced to the system,and a new mixed solvent system was prepared by adding acetic acid,dimethyl sulfoxide(DMSO)and several drops of Triton X-100TMto distilled water.CS/PEO(80/20)with the concentration of 3%was dissolved in the mixed solvent to prepare electrospinning solution for CS/PEO(80/20)nanofiber fabrication.The CS containing AgNPs electrospun solution could be prepared by replacing the distilled water to silver nanoparticle solution during the preparation of mixed solvent.Ultraviolet visible(UV-Vis)spectra and transmission electron microscope(TEM)results showed that silver nanoparticles were prepared successfully.CS membranes with and without AgNPs were acquired via a traditional electrospinning equipment.These two nanofiber membranes were characterized by scanning electron microscope(SEM)images and mechanical testing.It could be noticed from the SEM images that there was a good morphology and random distribution for the nanofibers with an average fiber diameter of 180 nm.The mechanical property results showed that the addition of AgNPs decreased the mechanical strength significantly but the mechanical strength could still support wound dressing application. Chitosan (CS) nanofibers were prepared by in-situ reducing method. A water soluble carboxymethyl chitosan (CMCT) was applied for the preparation of AgNPs. The impact factor such as the concentration of CMCT, silver nitrate (AgNO 3 ), temperature and the heating time were set as 0.1%, 20 μL AgNO 3 (1.7 mol / L ), 90 ° and 3 h, separately and the maximum concentration of AgNPs could be solved. To solve the spinnability of chitosan nanofiber, a super high molecular weight polyethylene oxide (PEO) was introduced to the system, and a new mixed solvent system was prepared by adding acetic acid, dimethyl sulfoxide (DMSO) and several drops of Triton X-100TMto distilled water. CS / PEO (80/20) with the concentration of 3% was dissolved in the mixed solvent to prepare electrospinning solution for CS / PEO (80/20) nanofiber fabr ication.The CS containing AgNPs electrospun solution could be prepared by replacing the distilled water to silver nanoparticle solution during the preparation of mixed solvent. Ultraviolet visible (UV-Vis) spectra and transmission electron microscope (TEM) results showed that silver nanoparticles were prepared successfully .CS membranes with and without AgNPs were acquired via a traditional electrospinning equipment. These two nanofiber membranes were characterized by scanning electron microscope (SEM) images and mechanical testing. It could be noticed from the SEM images that there was a good morphology and random distribution for the nanofibers with an average fiber diameter of 180 nm. The mechanical property results showed that the addition of AgNPs decreased the mechanical strength significantly but the mechanical strength could still support wound dressing application.