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本文对影响牛蛙胚胎发育的四种主要环境因素作了系统的研究。牛蛙胚胎发育的上限致死温度为36℃,下限温度为12℃,适宜温度为20~33℃,最适温度范围为26、30℃;在适宜温度范围内,随着温度的升高,胚胎发育速度加快,在水温30℃时,胚胎发育的速度比在20℃时快一倍。胚胎发育的不同阶段对水中溶氧量的要求不同,在心跳期以前,水中溶氧量必须保持在3.5mg/升以上;在鳃盖完成期以前应保持在5.1mg/升以上,29天以上的蝌蚪对溶氧的要求又比较低。孵化用水的pH值应保持在6.5~7.5的范围内。剧烈的机械振荡对早期胚胎有明显的不良影响。
This article systematically studies the four main environmental factors that affect the development of bullfrog embryos. The upper limit lethal temperature of the bullfrog embryo development is 36 ℃, the lower limit temperature is 12 ℃, the suitable temperature is 20 ~ 33 ℃, the optimum temperature range is 26, 30 ℃; in the suitable temperature range, with the temperature increasing, the embryo development Faster, in the water temperature 30 ℃, the embryo development faster than at 20 ℃ twice as fast. The different stages of embryonic development require different amounts of dissolved oxygen in water. Prior to the heartbeat period, the dissolved oxygen level in the water must be maintained above 3.5 mg / liter; it should be maintained above 5.1 mg / liter before the completion of the operculum and above 29 days Tadpoles on the dissolved oxygen requirements and relatively low. Incubation water should be maintained in the pH range of 6.5 to 7.5. Severe mechanical oscillations have significant adverse effects on early embryos.