由于未能早期诊断,使许多胰腺癌病人死于其疾病的晚期,设法早期检测出肿瘤疾患将改善病人的预后。有80％以上的胰腺癌病人的K-ras基因第12密码子位点上存在基因突变现象;检测十二指肠液中这些突变的基因,可以早期发现胰腺癌,并有助于早期诊断的确立。 有61例病人因患壶腹周围癌或胰腺的其他良性病变而实施胰十二指肠切除术(Whipple手术),从这些病人的十二指肠液中分离出DNA标本,用或不用 Due to the failure to make early diagnosis, many patients with pancreatic cancer die in the late stages of their disease. Trying to detect tumor diseases early will improve the patient’s prognosis. Over 80% of patients with pancreatic cancer have a gene mutation at the 12th codon site of the K-ras gene; detection of these mutations in duodenal fluid allows early detection of pancreatic cancer and contributes to the establishment of early diagnosis. . 61 patients underwent pancreatoduodenectomy (Whipple surgery) for peripheral periampullary cancer or other benign lesions of the pancreas. DNA samples were isolated from the duodenal fluid of these patients with or without