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为了进一步探讨胰岛淀粉样多肽（ＩＡＰＰ）的分布、定位以及它与其他生物活性物质的关系；用ＩＡＰＰ组织化学ＰＡＰ邻片双标法，观察了１８例１４～３８周人胎气管内ＩＡＰＰ免疫反应（ＩＲ）细胞的个体发生及与５羟色胺（５－ＨＴ）的关系。结果显示，胎１４周，气管粘膜表面的假复层柱状上皮中已有ＩＡＰＰ－ＩＲ细胞（Ｆｉｇ．１＆２）；１５周开始，粘膜固有层气管腺导管上皮中也出现分散的ＩＡＰＰ－ＩＲ细胞（Ｆｉｇ．３）；随胎龄增长，１７～２１周，气管上皮内ＩＡＰＰ－ＩＲ细胞逐渐增多；免疫染色加深（Ｆｉｇ．４＆５），有些细胞发出细突直达腔面（Ｆｉｇ．６＆７），粘膜下层的气管腺腺泡中也有ＩＡＰＰ－ＩＲ（Ｆｉｇ．８）； ２２～３８周，气管内 ＩＡＰＰ－ＩＲ 细胞又呈逐渐减少趋势，ＩＡＰＰ－ＩＲ仅出现在基底锥形细胞中（Ｆｉｇ．９＆１０），且免疫染色较深。邻片未显５－ＨＴ－ＩＲ。本研究表明，人胎儿期气管上皮细胞内有ＩＡＰＰ的表达；且ＩＡＰＰ－ＩＲ细胞随胎期的发育而发生变化。
In order to further explore the distribution and localization of islet amyloid polypeptide (IAPP) and its relationship with other bioactive substances, IAPP histochemical PAP adjacent double labeling method was used to observe the IAPP immunoreactivity in 18 cases of human fetal trachea 14 to 38 weeks (IR) cells and their association with serotonin (5-HT). The results showed that IAPP-IR cells were present in the pseudostratified columnar epithelium of the tracheal mucosa at 14 weeks (Fig.1 & 2). Dispersed IAPP-IR cells also appeared in the lamina propria of the lamina propria of the mucosa from the 15th week (Fig. 4 & 5). Some of the cells emitted fine axons directly to the luminal surface (Fig. 6 & 7), while the submucosa IAPP-IR also appeared in the tracheal gland acinar (Fig.8). In the period of 22-38 weeks, the IAPP-IR cells in the trachea also showed a decreasing trend. The IAPP-IR only appeared in the basal conical cells (Fig.9 & 10) And immunostaining deeper. Adjacent pieces did not show 5-HT-IR. This study shows that IAPP expression in human fetal tracheal epithelial cells; and IAPP-IR cells with fetal development changes.