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对大规模而迅速地规定抗寒性的若干方法进行了评定,其步骤如下:”1、与秋、冬、春三个季节的自然变化相应,对苗木进行耐寒锻炼和去耐寒锻炼的不同阶段预处理;2、按不同阶段对整个植株作不同程度的冷冻处理;3、然后检查树苗经冷冻试验后是否存活,以评定各种测定方法.茎干上部电阻抗比率的差异能预测冷冻后的存活情况,准确度达87%,亚能在耐寒锻炼和去耐寒锻炼的各个阶段测定出50%死亡的致死温度,范围不超过2℃.至于酶的活性、光合作用以及含水量变化等的测定,效应都依次减小,冬期尤为明显.应用多重判别分析得出合并变量,略可提高上述测定效应.应用温度梯度杆(temperature gradient bar),测定离体器官的抗寒性,不必测定整个植株,可以进一步缩短抗寒性鉴别程序所花的时间.
A number of methods for determining large-scale and rapid cold hardiness were evaluated. The steps are as follows: "1. Corresponding to the natural changes in the autumn, winter and spring seasons, different stages of cold-resistant seedlings and cold- Pretreatment 2, freezing the whole plant at different stages according to different stages 3, and then checking whether the saplings survived the freezing test to evaluate various methods of measurement The difference in the electrical resistance ratio of the upper stem can predict the post- Survival, accuracy of 87%, Asia can in cold-resistant exercise and to all stages of cold-resistant exercise to determine the lethal temperature of 50% death, the range does not exceed 2 ° C. As for the activity of the enzyme, photosynthesis and water content change determination , The effects are reduced in turn, especially in winter.Using multi-discriminant analysis of the merger variables, can slightly improve the above determination effect.Using temperature gradient bar (temperature gradient bar) to measure the cold resistance of isolated organs, without measuring the entire plant , Can further shorten the identification of cold resistance time spent.