论文部分内容阅读
目的:建立一种简便、快速、可靠的检测A-to-IRNA编辑酶活性的方法。方法与结果:一步法制备的C57BL/6小鼠十种组织的全组织提取物,在各提取物中检测到A-to-IRNA编辑酶的非特异性编辑活性,不同组织中A-to-IRNA编辑酶活性的强度依次为脑>肺>胸腺>脾>淋巴结>肝>肾>睾丸>心脏>骨骼肌,编辑活性与加入反应体系中的蛋白量成正相关。结论:一步法制备的全组织提取物可用于检测A-to-IRNA编辑酶活性,该方法操作简便、可控、省时。
Objective: To establish a simple, rapid and reliable method for the detection of A-to-IRNA editing enzyme activity. Methods and Results: Ten tissues of C57BL / 6 mice were prepared by one-step method. The non-specific editing activity of A-to-IRNA editing enzyme was detected in each extract. The A-to-IRNA The order of activity of editing enzymes was brain> lung> thymus> spleen> lymph node> liver> kidney> testis> heart> skeletal muscle. The activity of editing activity was positively correlated with the amount of protein added to the reaction system. Conclusion: The whole tissue extracts prepared by one-step method can be used to detect A-to-IRNA editing enzyme activity. The method is simple, controllable and time-saving.