论文部分内容阅读
本文实验采用FMT-182型γ免疫计数器,利用放射免疫法对4组不同时间肠缺血再灌注犬(对照组,绞窄2小时组,绞窄4小时组和绞窄6小时组)的血清进行检测,探讨机体自身超氧化物歧化酶(SOD)的改变。实验发现,肠绞窄2小时再灌注组其SOD变化呈上升趋势,而绞窄4小时和6小时再灌注组其SOD表现为下降。各组之间SOD改变有显著差异(P<0.05)。与对照组相比,绞窄4小时组和绞窄6小时组动物在术后4小时和8小时其SOD下降显著(P<0.05和P<0.01)且病理损害明显。由于SOD是氧自由基的清除剂,通过测定其活性可间接了解O_2~-等自由基对肠组织缺血病灶的影响,对判断预后有一定帮助。
In this study, FMT-182 gamma immune counters were used to administer radioimmunoassay to 4 groups of dogs with different durations of intestinal ischemia and reperfusion (control group, strangulation for 2 hours, strangulation for 4 hours and strangulation for 6 hours). The test was conducted to investigate the changes in the body’s own superoxide dismutase (SOD). It was found that the changes in SOD in the ileus group 2 hours after ileus tend to increase, while the SOD in the 4 hours and 6 hours reperfusion group decreased. There was a significant difference in SOD between groups (P<0.05). Compared with the control group, the SOD decreased significantly (P<0.05 and P<0.01) at 4 hours and 8 hours after operation in the 4-wk group and 6-hour strangulation group, and the pathological damage was significant. Since SOD is a scavenger of oxygen free radicals, the effect of O2~- and other free radicals on the ischemic lesions of the intestine can be indirectly understood by measuring the activity of SOD, which is helpful for judging the prognosis.