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目的本实验旨在探究鹿茸对孕鼠和胎鼠的影响。方法昆明小鼠随机分组,A组:♀48只,♂24只以♂:♀=1∶2的比例合笼,孕第5天～第14天连续对雌鼠经口灌胃给3 mg.kg-1 d-1、6 mg.kg-1 d-1和12 mg.kg-1 d-1剂量的鹿茸;B组:♀96只,♂48只,雄鼠连续给鹿茸(剂量同A组雌鼠)60 d后,以♂∶♀=1∶2的比例合笼,从受孕率、孕鼠体重、死胎率、吸收胎率、胎盘重、子宫和胎鼠重以及胎鼠的外观、内脏和骨骼等方面进行检查。结果 A组:在第18天时,高剂量组体质量、死胎率、吸收胎率、胎鼠外观、内脏和骨骼畸形率与对照组比较,具有统计学意义(P<0.05);随剂量的升高,胎盘重、子宫和胎鼠重有所降低,但没有统计学意义。B组:中剂量组与对照组之间比较,受孕率显著增加,具有统计学意义(P<0.05);随着剂量的增加各组死胎率、吸收胎率、胎盘重、子宫和胎鼠重无显著差异(P>0.05);各给药组与对照组比较,对胎鼠畸形没有造成影响。结论在本实验剂量范围内,高剂量组鹿茸在雌鼠妊娠期间可导致孕鼠体质量下降,死胎率、吸收胎率增加;胎鼠外观、内脏和骨骼畸形增加;中剂量鹿茸在交配前对雄鼠灌胃可提高受孕率,对胎鼠不造成畸形。
Objective The purpose of this experiment is to investigate the effects of antler on pregnant rats and fetus. Methods Kunming mice were randomly divided into groups A and B: 只 48, ♂ 24 were caged with the ratio of ♂: ♀ = 1: 2. The rats were orally gavaged with 3 mg for the first 5 days to the 14th day of pregnancy. kg-1 d-1, 6 mg.kg-1 d-1 and 12 mg.kg-1 d-1. The rats in group B: ♀96, ♂48, Group of female rats) 60 days later, the ratio of ♂:♀ = 1: 2 was taken from the cage, from the pregnancy rate, body weight of pregnant rats, stillbirth rate, absorption rate, placental weight, uterus and fetal rat weight, Viscera and bones and other aspects of inspection. Results In group A, body weight, stillbirth rate, absorbed fetal rate, appearance of fetus, visceral and skeletal deformity rate in high-dose group were statistically significant compared with those in control group on day 18 (P <0.05) High, placental weight, uterus and fetal rat weight decreased, but not statistically significant. Group B: Compared with the control group, the conception rate was significantly increased (P <0.05); with the dose increasing, the stillbirth rate, the ratio of the fetus, the weight of the placenta, the weight of the uterus and the fetal rat No significant difference (P> 0.05); each administration group compared with the control group, did not affect the fetus deformity. Conclusions In the experimental dose range, the high-dose antler reduced the body weight, the stillbirth rate and the absorption rate of the fetus during gestation, and increased the appearance, visceral and skeletal malformations of the fetus; Male rats gavage can increase the rate of pregnancy, the fetus does not cause deformity.