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目的研究结外NK/T细胞淋巴瘤(ENKTCL)组织中c-MYB基因及microRNA-150基因的表达水平及其相关性。方法收集本院2010年1月至2014年6月经病理组织学及免疫组织化学证实且临床资料完全的ENKTCL病理标本21例,10例增生性淋巴结炎作为对照。用免疫组织化学Maxvision法和实时荧光定量PCR法,分别检测21例ENKTCL和10例增生性淋巴结炎组织中c-MYB蛋白和microRNA-150表达水平,并进行分析与比较。结果 ENKTCL组c-MYB表达率为57%,显著高于增生性淋巴结炎组10%(P<0.05),但c-MYB过表达与患者的年龄、性别、Ann Arbor分期、国际预后指数评分无关(P>0.05)。ENKTCL组织中MicroRNA-150表达与c-MYB表达呈显著负相关(P<0.05)。结论 MicroRNA-150可能通过靶向调控c-MYB基因表达参与了ENKTCL的发生与发展。
Objective To study the expression of c-MYB gene and microRNA-150 gene in the extranodal NK / T cell lymphoma (ENKTCL) and its correlation. Methods Twenty-one ENKTCL pathological specimens confirmed by histopathology and immunohistochemistry from January 2010 to June 2014 in our hospital were collected, and 10 cases of proliferative lymphadenitis as control. The expression of c-MYB protein and microRNA-150 in 21 cases of ENKTCL and 10 cases of proliferative lymphadenitis were detected by immunohistochemistry Maxvision method and real-time fluorescence quantitative PCR method, and analyzed and compared. Results The c-MYB expression rate in ENKTCL group was 57%, which was significantly higher than that in 10% (P <0.05) of proliferative lymphadenitis group. However, the c-MYB overexpression was not related to the patient’s age, gender, Ann Arbor stage and International Prognostic Index (P> 0.05). MicroRNA-150 expression in ENKTCL tissues was negatively correlated with c-MYB expression (P <0.05). Conclusion MicroRNA-150 may participate in the occurrence and development of ENKTCL by targeting the regulation of c-MYB gene expression.