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研究了以霍乱毒素Ｂ亚基（ＣＴＢ）为载体的重组疟疾多价抗原（ＡＷＴＥ）表位的ＤＮＡ疫 苗在恒河猴中的免疫原性及对相应疟原虫感染的免疫保护作用。结果表明：ＤＮＡ疫苗组免疫 ２次后即产生了较高水平的细胞免疫和体液免疫，免疫后９１天用 １．２５ ×１０８个食蟹疟原虫攻 击，对照组５只动物在攻击后１４天左右全部感染，感染持续３４天以上；ＤＮＡ疫苗组的５只动 物一直到攻击后６０天，没有感染。另外，还检测了免疫后不同时间各组的免疫应答水平，与对 照组相比，ＤＮＡ疫苗组免疫２次后即产生了较高水平的细胞免疫和体液免疫。从实验结果来 看，首先说明了选择的这种鸡尾酒式的抗原表位组合构建的ＤＮＡ疫苗具有很好的免疫原性， 同时也说明了ＤＮＡ疫苗在抗疟感染中起着举足轻重的作用。
To investigate the immunogenicity and immune protection of DNA vaccine against Rhesus malaria infection in Rhesus macaques using the cholera toxin B subunit (CTB) as carrier of recombinant malaria polyvalent antigen (AWTE) epitope. The results showed that the DNA vaccine group produced a high level of cellular immunity and humoral immunity after immunized twice, 1.25 × 108 P. melilotis 91 days after immunization, and 5 animals in control group on the 14th day after challenge All the infections were infected with the infection for more than 34 days. Five animals in the DNA vaccine group were not infected until 60 days after the challenge. In addition, the immune response of each group at different times after immunization was also tested. Compared with the control group, the DNA vaccine group produced higher levels of cellular immunity and humoral immunity after being immunized twice. The experimental results show that the DNA vaccine constructed by combining the selected cocktail epitopes has good immunogenicity and at the same time shows that DNA vaccines play an important role in anti-malarial infection.