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为了探讨恶性肿瘤转移与微核现象间的关系,本实验首次运用微核试验(MT)检测了小鼠腹水型肝癌(N_(22))及其肺转移倾向亚株(H_(22)SP_(10))、淋巴结转移倾向亚株(N_(22)SL_(10))。小鼠子宫颈癌(U_(14))及其肺转移倾向亚株(U_(14)AP_(11))瘤细胞内的微核。比较了经活体筛选的上述亚株与未经筛选的原瘤株的微核细胞率(MNF)、总微核率,结果上述两项指标H_(22)SP_(10)及H_(22)SL_(10)均显著高于H_(22)(P值分别为<0.002、<0.005;<0.005、<0.001);U_(14)AP_(11)也显著高于U_(14)(P分别为<0.001、<0.001),而H_(22)SL_(10)与H_(22)SP_(10)(P>0.05、>0.05)。它表明在异质性N_(22)及U_(14)细胞群体中具有器官倾向性转移的细胞染色体畸变较重,可能为肿瘤演进的结果。
In order to investigate the relationship between malignant tumor metastasis and micronucleus phenomenon, this experiment first used micronucleus test (MT) to detect mouse ascites type liver cancer (N_(22)) and its subtropical metastasis-prone strain (H_(22)SP_( 10)) Lymph node metastasis sub-strain (N_(22)SL_(10)). Micronuclei in Murine Cervical Carcinoma (U_(14)) and Its Lung Metastasis-Prone Sub-Strain (U_(14)AP_(11)) Tumor Cells. The micronuclear cell rate (MNF) and total micronucleus rate of the above-mentioned sub-strains and non-screened proto-tumor strains were compared. The results were as follows: H_(22)SP_(10) and H_(22)SL_. (10) Significantly higher than H_(22) (P values were <0.002, <0.005, <0.005, <0.001); U_(14)AP_(11) was also significantly higher than U_(14) (P were < 0.001, <0.001), while H_(22)SL_(10) and H_(22)SP_(10) (P>0.05, >0.05). It suggests that chromosome heterogeneity in organs with heterozygous N_(22) and U_(14) cell metastases is severe and may be the result of tumor progression.