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借鉴文献方法,我们以小鼠L_(1210)白血病细胞为村料,提取DNA拓扑异构酶Ⅱ,鉴别了其生物学特性,并观察了十多种已知及未知的抗癌药物对其活性的影响,初步建立了以DNA拓扑异构酶Ⅱ为靶点的新抗癌药筛选方法。实验表明DNA拓扑异构酶Ⅱ对DNA链切割反应是可逆的,并且高浓度的氯化钠对其活性有抑制作用。许多药物能促进拓扑酶Ⅱ引起的DNA链断裂如ADM、DNR、Vp16及ACM—B等,而另一些药物如萜类化合物BC_1、BC_4等则能抑制链断裂反应。
By referring to the literature method, we used mouse L_(1210) leukemia cells as the source material to extract DNA topoisomerase II, identified its biological characteristics, and observed the activity of more than 10 known and unknown anticancer drugs. The impact of the preliminary establishment of the DNA topoisomerase II as a target for screening of new anticancer drugs. Experiments show that DNA topoisomerase II is reversible to DNA strand cleavage reaction, and high concentration of sodium chloride has inhibitory effect on its activity. Many drugs can promote DNA strand breaks caused by topoisomerase II such as ADM, DNR, Vp16, and ACM-B, while other drugs such as steroids BC_1, BC_4, etc. can inhibit the chain cleavage reaction.