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基因工程的应用在阐明乙型肝炎病毒感染的性质中是不可缺少的,因为现在还没有一种细胞培养系统能繁殖乙型肝炎病毒(HBV),同时,HBV的研究是临床上一个主要的课题。第一步是在大肠杆菌中无性繁殖HBV的DNA,并制备大量的纯病毒DNA,作物理分析。3种亚型的全部序列得出后,应作相互比较,这样才能对DNA上4个可能编制密码的区域进行定位。其中之一已证明为相当于基因S,即多肽PI的密码基因,而多肽PI是病毒外壳的主要蛋白成分,即HBs的主要成分。第二个编制密码区相当于基因,即编制多肽P19的密码,
Genetic engineering is indispensable in elucidating the nature of hepatitis B virus infection because no cell culture system is capable of producing Hepatitis B virus (HBV), and the study of HBV is a clinically important issue . The first step is to asexually propagate HBV DNA in E. coli and prepare a large amount of pure viral DNA for physical analysis. After all the sequences of the three subtypes have been derived, they should be compared with each other so that the four areas in the DNA where the code may be coded can be located. One of these has been shown to be the equivalent of the gene S, the codon for the polypeptide PI, which is the major protein component of the virus’s coat, the main component of HBs. The second coding area is equivalent to the gene, that is, the preparation of peptide P19 password,