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1细胞色素C的进一步纯化1.1原理哺乳动物心肌细胞色素C是一种强碱性聚电解质(等电点>pH10),经弱酸性阳离子交换剂如Amberlite IRC-50(聚羧基树脂)或Decalso-F(硅酸铝)吸附后可易被洗脱。与细胞色素C制品相关的非细胞色素蛋白则不被阳离子交换剂吸附,因此容易与细胞色素C分离。
1 Further Purification of Cytochrome C 1.1 Principles Mammalian cardiomyocytes Cytochrome C is a strongly basic polyelectrolyte (isoelectric point> pH 10) that is neutralized by weakly acidic cation exchangers such as Amberlite IRC-50 (polycarboxylate resin) or Decalso- F (aluminum silicate) can be easily eluted after adsorption. Cytochrome C products associated with non-cytochrome protein is not adsorbed by the cation exchanger, and therefore easy to separate from cytochrome C.