论文部分内容阅读
目的:比较黄芩生品、炭品止血效果,筛选黄芩炭有效止血部位,通过血热出血动物模型探讨该部位发挥止血作用的机制。方法:取SD大鼠60只,随机分为6组,空白对照组、模型对照组、云南白药组、黄芩生品组、黄芩炭品组、黄芩炭氯仿部位组。将供试品按每日20 mL.g-1的剂量灌胃(ig),1次/d,给药7 d。通过测定其凝血酶原时间(PT)、凝血酶时间(TT)、活化部分凝血活酶时间(APTT)、血浆纤维蛋白(FIB)4项指标及血液流变学相关指标,观察黄芩生品、炭品及各部位对模型动物血液系统的影响。结果:模型对照组较空白对照组有显著差异,显著延长PT(17.26±1.73)s,TT(23.15±1.83)s,APTT(22.43±1.93)s及高切黏度(5.72±0.55)mPa.s,中切黏度(7.44±0.78)mPa.s,低切黏度(6.59±1.10)mPa.s。黄芩炭和黄芩炭氯仿部位能显著缩短血热出血动物模型的PT,TT,APTT。黄芩炭和黄芩炭氯仿部位依次为PT(14.53±1.45)s,(13.24±1.46)s;TT(20.91±1.15)s,(18.70±1.44)s;APTT(18.07±0.75)s,(15.59±1.74)s;与模型对照组比较P<0.05或P<0.01;对FIB均没有显著影响。结论:黄芩炭氯仿部位为黄芩炭止血的有效部位,止血主要通过内源性、外源性凝血酶及内外源共同途径,与纤维蛋白系统无关。血液流变学的改善为止血效果的产生提供依据。
OBJECTIVE: To compare the hemostatic effect of Scutellaria baicalensis and charcoal, and to screen the effective hemostatic sites of Scutellaria baicalensis Georgi, to explore the mechanism of hemostasis exerted by this site through the animal model of hemorrhagic fever with blood-heat. Methods: Sixty SD rats were randomly divided into 6 groups, blank control group, model control group, Yunnan Baiyao group, Huangqin group, Huangqin group and Huangqin group. The test will be daily doses of 20 mL.g-1 gavage (ig), 1 / d, administered 7 d. Scutellaria baicalensis Georgi were observed by measuring 4 indexes of prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (APTT), plasma fibrin (FIB) Effects of carbon products and various parts on the blood system of model animals. Results: Compared with the blank control group, the model control group significantly prolonged PT (17.26 ± 1.73) s, TT (23.15 ± 1.83) s, APTT (22.43 ± 1.93) s and high shear viscosity (5.72 ± 0.55) mPa.s (7.44 ± 0.78) mPa.s and low shear viscosity (6.59 ± 1.10) mPa.s. Scutellaria baicalensis and Scutellaria carbon chloroform site can significantly shorten the blood-heat animal model of PT, TT, APTT. The carbon and chloroform fractions of Scutellaria baicalensis and Scutellaria baicalensis lie in the order of PT (14.53 ± 1.45) s, (13.24 ± 1.46) s, TT (20.91 ± 1.15) s, (18.70 ± 1.44) s and APTT 1.74) s; compared with the model control group, P <0.05 or P <0.01; no significant effect on FIB. CONCLUSION: The chloroform-chloroform fraction of Radix Scutellariae is an effective site for the hemostasis of Scutellaria baicalensis Georgi, and the hemostasis is mainly independent of the fibrin system through the common pathway of endogenous and exogenous thrombin and endogenous and exogenous sources. Hemorheological improvement to provide the basis for the hemostatic effect.