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目的检测miR-451在肾癌患者血清中的表达,研究miR-451与肾癌患者临床病理特征之间的关联性。方法收集35例新诊断肾癌患者术前血清样本,另外收集35例健康体检者血清样本做为对照,利用实时荧光定量PCR技术检测miR-451在肾癌患者血清中的表达情况,分析肾癌患者临床病理特征和miR-451表达量两者之间的关系。结果与对照组比较,肾癌组患者血清中miR-451表达量明显低于健康体检组,差异有统计学意义(P<0.05),在35例肾癌患者血清样本中,27例(77.14%)miR-451下调,而8例(22.86%)则表现为上调。肾癌患者的性别、年龄、肾癌组织类型、肾肿瘤大小、TNM分期和肾癌患者血清中miR-451表达量无相关性(P>0.05)。结论 miR-451在肾癌患者血清中低表达,提示其可能与肾癌的发生和发展相关。
Objective To detect the expression of miR-451 in the serum of patients with renal cell carcinoma and to study the correlation between miR-451 and clinicopathological features of patients with renal cell carcinoma. Methods 35 cases of newly diagnosed patients with renal cell carcinoma were collected preoperative serum samples, another 35 healthy subjects were collected serum samples as a control, the use of real-time fluorescence quantitative PCR detection of miR-451 in renal cell carcinoma serum expression, analysis of renal cell carcinoma The relationship between the clinicopathological features and the expression level of miR-451 in patients. Results Compared with the control group, the expression of miR-451 in the serum of patients with renal cell carcinoma was significantly lower than that of the healthy control group (P <0.05), and in the serum samples of 35 patients with renal cell carcinoma, 27 (77.14% ) miR-451 downregulation, while 8 cases (22.86%) showed up-regulation. There was no correlation between gender, age, type of renal cell carcinoma, size of renal tumor, TNM stage and serum miR-451 expression in patients with renal cell carcinoma (P> 0.05). Conclusion The low expression of miR-451 in the serum of patients with renal cell carcinoma suggests that it may be related to the occurrence and development of renal cell carcinoma.