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目的:探讨罗布麻叶提取物对两肾一夹加高糖高脂饮食大鼠脂肪性肝病的影响.方法:采用两肾一夹术和喂高糖高脂饲料12wk复制代谢综合征大鼠脂肪性肝病模型,第5周测定大鼠收缩压,取收缩压达160mmHg以上的大鼠42只,随机分为罗布麻叶提取物高、中、低剂量组[200、100、50mg/(kg·d),n=8]、卡托普利组[30mg/(kg·d),n=8]和模型组[0.5%羧甲基纤维素5mg/(kg·d),n=10],另设假手术组[0.5%羧甲基纤维素5mg/(kg·d),n=10],按上述设定剂量每天9:00-10:00灌胃一次连续8wk.第13周,称体质量和肝湿质量,测收缩压、血清胰岛素和FFA水平、肝功能、肝组织氧化和抗氧化指标,HE染色观察肝组织形态学改变.结果:模型组大鼠血清胰岛素、FFA、GOT和GPT水平明显升高,肝组织中T-SOD和GSH-Px活力和ISI明显下降,脂质过氧化物MDA含量显著增加,HE染色示肝小叶中大部分肝细胞肿胀,胞质中出现大小不等的脂滴空泡,以大泡性脂肪滴为主,核居边,细胞界限不清,肝窦狭窄,且存在小叶内及门管区炎症细胞浸润.与模型组比较,罗布麻叶提取物高中剂量能明显降低代谢综合征大鼠血清胰岛素、FFA、GOT和GPT水平(41.31±14.46,48.03±10.19vs62.56±19.48;468.52±205.36,584.14±185.89vs830.51±355.17;192.63±67.41,325.18±96.47vs466.16±95.63;49.06±21.52,108.32±38.63vs178.37±66.55,P<0.05或0.01),提高肝组织中T-SOD和GSH-Px活力以及ISI,减少脂质过氧化物MDA含量(83.80±7.10,70.86±11.68vs47.33±12.53;681.85±173.99,481.27±70.05vs250.50±102.97;-5.19±0.44,-5.45±0.31vs-5.79±0.42;17.36±3.45,25.69±5.46vs37.69±4.13,P<0.05或0.01),改善肝细胞的脂质沉积和小叶内及汇管区炎症细胞浸润.结论:罗布麻叶提取物具有抗氧化应激和改善代谢综合征大鼠脂肪性肝病的作用.
Objective: To investigate the effect of Apocynum venetum extract on fatty liver disease in rats with two nephrons plus hyperglycaemia and hyperlipidemia.Methods: Two-kidney-one-clip and high-fat and high-fat diet fed with 12-week-old rat fat metabolism syndrome In the fifth week, 42 rats with systolic blood pressure more than 160mmHg were randomly divided into high, middle and low dose groups of Apocynum venetum extract [200, 100, 50 mg / (kg · captopril group [30mg / (kg · d), n = 8] and model group [0.5% carboxymethylcellulose 5mg / (kg · d), n = 10] Another set of sham operation group [0.5% carboxymethylcellulose 5mg / (kg · d), n = 10], according to the above set dose daily gavage 9: 00-10: 00 continuous 8wk week 13, said The body weight and liver wet mass, systolic blood pressure, serum insulin and FFA levels, liver function, liver tissue oxidation and anti-oxidation index, liver tissue morphology were observed by HE staining.Results: The levels of insulin, FFA, GOT and GPT levels were significantly increased, T-SOD and GSH-Px activity and ISI in liver tissue decreased significantly, MDA content of lipid peroxides increased significantly, most of hepatocytes in hepatic lobules were swollen by HE staining, Such as lipid droplets vacuoles Foam fat droplets, nucleus edge, cell boundaries unclear, sinusoidal stenosis, and the presence of lobular and portal inflammatory cell infiltration.Compared with the model group, Apocynum leaves extract high school dose can significantly reduce the metabolic syndrome Serum insulin, FFA, GOT and GPT levels (41.31 ± 14.46, 48.03 ± 10.19 vs. 62.56 ± 19.48; 468.52 ± 205.36, 584.14 ± 185.89 vs 830.51 ± 355.17; 192.63 ± 67.41, 325.18 ± 96.47 vs 466.16 ± 95.63; 49.06 ± 21.52,108.32 ± 38.63vs178.37 ± 66.55, P <0.05 or 0.01), increased T-SOD and GSH-Px activities and ISI in liver tissue, decreased lipid peroxidation MDA content (83.80 ± 7.10, 70.86 ± 11.68vs47.33 ± 12.53; 681.85 ± 173.99,481.27 ± 70.05vs250.50 ± 102.97; -5.19 ± 0.44, -5.45 ± 0.31vs-5.79 ± 0.42; 17.36 ± 3.45,25.69 ± 5.46vs37.69 ± 4.13, P < 0.05 or 0.01), and improved the lipid deposition of hepatocytes and infiltration of inflammatory cells in the lobes and portal area.Conclusion: Apocynum venetum extract has anti-oxidative stress and improves the fatty liver disease in rats with metabolic syndrome.