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目的:通过佛波酯诱导内皮细胞增生体外模拟银屑病新生的病理状态,比较养血活血解毒方及其组分药物血清对人皮肤微血管内皮细胞及相关信号转导通路的干预效应。方法:佛波酯诱导内皮细胞24 h造模,10%中药含药血清作用细胞24 h。采用CCK-8比色法检测全方及拆方药物血清对内皮细胞增殖的影响;Transwell小室法检测药物血清对细胞迁移的影响;Ma-
OBJECTIVE: To observe the pathological state of neonatal psoriasis induced by phorbol ester in vitro, and to compare the intervention effects of nourishing blood, activating blood and removing detoxification prescription and its components of serum on human dermal microvascular endothelial cells and related signal transduction pathways. Methods: Epirubicin induced endothelial cells 24 h modeling, 10% Chinese medicine serum-containing cells for 24 h. CCK-8 colorimetric assay was used to detect the effects of serum of all prescriptions and demolition prescriptions on the proliferation of endothelial cells. Transwell chamber assay was used to detect the influence of serum on cell migration. Ma-