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在离体培养条件下，芝麻（ｌ３ＯｅｈｍｅｒｉａｎｉｖｅａＬ。）经秋水仙素处理，再经不定芽诱导和继代培养后，获得的变异株染色体数目出现多样性，其中包括四倍体、三倍体、二倍体和多种非整倍体细胞。以四倍体和三倍体水平的植株为母本与二倍体栽培品种杂交，对杂交后代进行倍性观察，发现染色体数目变化在１２－８４之间。不同植株甚至同一植株染色体数目也不一致。从２５１个杂交后代中获得的三倍体细胞所占比例超过５０％的植株有３５个。还对多倍体水平的植株进行了有丝分裂观察，发现三极分裂、不均等分裂和落后染色体等异常有丝分裂现象。其次，还观测了气孔、花粉粒，同样发现多样性。
Under the condition of in vitro culture, sesame (l3OehmerianiveaL.) Treated with colchicine, and then by adventitious bud induction and subculture, the number of chromosomes obtained diversity, including tetraploid, triploid, two Ploidy and a variety of aneuploid cells. The tetraploid and triploid plants were crossed with diploid cultivars, and the ploidy of hybrid progenies was observed. The number of chromosomes varied between 12 and 84. Different plants and even the same plant chromosome number is not consistent. Thirty-five out of 251 hybridized offspring had more than 50% triploidy plants. Polyploid plants were also observed for mitosis and found abnormal mitosis such as bipolar division, unequal division, and lagging chromosomes. Second, observations of stomata and pollen grains also revealed diversity.