为研究RAB5A基因对肺腺癌细胞中微丝的影响,通过FITC标记的鬼笔环肽对AGZY83a细胞骨架中的微丝特异染色,利用共聚焦激光扫描显微镜发现RAB5A过表达后微丝束变密。经Superarray肿瘤转移相关基因微芯片分析RAB5A对肿瘤转移相关基因的表达影响,发现了3个与细胞骨架调节相关基因表达发生变化,NM23H1与Rac1的表达受到抑制,同时S100A4的表达增加。以前有研究认为S100A4基因可抑制NM23H1基因表达,为验证NM23H1基因的表达降低是否由于S100A4表达增高所致,利用RNAi沉默AGZY83a细胞中S100A4基因的表达,发现NM23H1基因表达增高,由此推断RAB5A基因可能通过上调S100A4基因表达来抑制NM23H1基因表达。 In order to study the effect of RAB5A gene on the microfilaments in lung adenocarcinoma cells, the FITC-labeled phalloidin was used to stain the filaments in AGZY83a cytoskeleton. Confocal laser scanning microscope . The effect of RAB5A on the expression of tumor metastasis-related genes was analyzed by Superarray micrometastatistical microchips. The expressions of three genes related to cytoskeleton regulation were found to be changed, the expressions of NM23H1 and Rac1 were inhibited, and the expression of S100A4 was also increased. Previous studies suggest that S100A4 gene can inhibit NM23H1 gene expression, in order to verify whether the reduced expression of NM23H1 gene is due to the increased expression of S100A4 RNAi silencing AGZY83a cells S100A4 gene expression and found that NM23H1 gene expression increased, suggesting that RAB5A gene may be Inhibition of NM23H1 gene expression by upregulation of S100A4 gene expression.