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目的:观察脑出血患者CSF和血清中S100β蛋白含量的变化及其临床意义。方法:以25例腹股沟疝和大隐静脉曲张患者作为25例脑出血患者对照组,取CSF和血清;将24只家兔随机分为两组(每组12只),一组实验组,另一组为假手术组。分别于实验性脑出血后6 h、12 h、24 h、48 h、72 h、96 h等各时间点取各组CSF和血清,采用ELISA法测定S100β蛋白的含量。结果:脑出血患者急性期与恢复期脑脊液中S100β蛋白的水平明显高于对照组(P<0.01);也明显高于血清S100β蛋白的水平(P<0.01);不同时间点兔脑出血模型实验组脑脊液中S100β蛋白的水平明显高于假手术组(P<0.01)。结论:脑出血后S100β蛋白在脑脊液中持续时间较长,且含量显著增高,可作为出血性脑损伤的早期诊断、指导治疗、判断预后的检测指标。
Objective: To observe the changes of S100β protein in CSF and serum of patients with intracerebral hemorrhage and its clinical significance. Methods: Twenty-five patients with inguinal hernia and saphenous varicose vein were selected as the control group of 25 patients with cerebral hemorrhage. CSF and serum were collected. Twenty-four rabbits were randomly divided into two groups (n = 12), one experimental group, One group was sham operation group. CSF and serum of each group were taken at different time points after 6h, 12h, 24h, 48h, 72h, 96h after experimental intracerebral hemorrhage. The content of S100β protein was determined by ELISA. Results: The levels of S100β protein in cerebrospinal fluid in acute and convalescent patients with cerebral hemorrhage were significantly higher than those in control group (P <0.01) and significantly higher than those in serum S100β (P <0.01). The intracerebral hemorrhage model at different time points The level of S100β in cerebrospinal fluid was significantly higher in sham operation group than in sham operation group (P <0.01). Conclusion: The S100β protein in cerebrospinal fluid after cerebral hemorrhage has a longer duration and a significantly higher content, which can be used as an early diagnosis and guide treatment for hemorrhagic brain injury to determine prognosis.