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目的 探讨乙型肝炎 (乙肝 )病毒数量在乙肝发病中的作用。方法 分别用竞争性聚合酶链反应技术、双抗体夹心酶联免疫法 (ELISA)和放射免疫分析法 (RIA) ,检测 2 41例各型乙肝患者和 2 0例正常人的血清HBVDNA浓度、血清可溶性白细胞介素 2受体 (sIL 2R)和肿瘤坏死因子(TNF)水平 ,进行相关分析。结果 急、慢性乙肝患者血清HBVDNA浓度的变化与sIL 2R(r≥0 6 979,P <0 0 5 ) ,TNF(r≥ 0 7782 ,P <0 0 5 )水平呈显著正相关 ;而重症乙肝、肝炎后肝硬化患者HBVDNA与sIL 2R(r≤ 0 2 96 3 ,P >0 0 5 ) ,TNF(r≤ 0 3987,P >0 0 5 )无相关性。结论 急、慢性乙肝患者细胞因子sIL 2R、TNF与体内HBV复制程度密切相关 ,提示抗病毒在急、慢性乙肝治疗中是一个积极有效的措施
Objective To investigate the role of hepatitis B virus (HBV) in the pathogenesis of hepatitis B. Methods Serum HBVDNA concentrations were detected by competitive polymerase chain reaction, double antibody sandwich enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) in 241 cases of various types of hepatitis B patients and 20 normal individuals. Serum Soluble interleukin 2 receptor (sIL 2R) and tumor necrosis factor (TNF) levels, were analyzed. Results Serum HBVDNA levels in acute and chronic hepatitis B patients were significantly correlated with serum levels of sIL 2R (r ≥ 0 6 979, P 0 05) and TNF (r ≥ 0 7782, P 0 05) There was no correlation between HBVDNA and sIL 2R (r ≤ 0 2 96 3, P 0 05) and TNF (r ≤ 0 3987, P 0 05) in patients with posthepatitic cirrhosis. Conclusions The levels of cytokine sIL 2R and TNF in patients with acute and chronic hepatitis B are closely related to the degree of HBV replication in vivo, suggesting that antiviral therapy is an active and effective measure in the treatment of acute and chronic hepatitis B