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采用新型Ca~(2+)荧光指示剂Fura-2建立双波长法测定兔MCAO局灶脑缺血后脑片神经细胞内游离钙([Ca~(2+)]_i),结果显示脑缺血后神经元[Ca~(2+)]_i明显升高。
A new Ca 2+ fluorescent indicator, Fura-2, was used to establish a dual wavelength method for the determination of intracellular free calcium ([Ca ~ (2 +)] i in rat brain after focal cerebral ischemia in MCAO. The results showed that cerebral ischemia After the neurons [Ca ~ (2 +)] _i significantly increased.