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目的探讨自由基在胃癌及其癌前病变发生中的作用．方法将１００只Ｗｉｓｔａｒ大鼠分为２组，实验组（７０只），给予１００ｍｇ／Ｌ甲基硝基亚硝基胍（ＭＮＮＧ）水溶液自由饮用３０ｗｋ，对照组（３０只）饮用自来水．选５个时相点，动态观察ＭＮＮＧ诱发实验性胃癌及其癌前病变过程中大鼠体内丙二醛（ＭＤＡ）、脂质过氧化物（ＬＰＯ）、谷胱甘肽过氧化物酶（ＧＳＨＰＸ）及超氧化物歧化酶（ＳＯＤ）等的变化情况．结果在实验组，ＭＤＡ平均含量在５２ｗｋ非常显著地大于０ｗｋ（Ｐ＜００１），并显著地大于１６ｗｋ以前（Ｐ＜００５）．胃癌组织ＭＤＡ含量显著高于胃癌癌前病变组织（Ｐ＜００５）．癌组织ＬＰＯ的含量显著高于癌前病变组织（Ｐ＜００５）．实验组，总ＳＯＤ和ＣｕＺｎＳＯＤ活性在５２ｗｋ明显低于１６ｗｋ之前（分别为Ｐ＜００５和Ｐ＜００１）．癌组织ＣｕＺｎＳＯＤ含量非常显著地小于正常胃粘膜（Ｐ＜００１），亦明显低于胃粘膜异型增生和肠上皮化生（Ｐ＜００５）．在３０ｗｋ和５２ｗｋＧＳＨＰＸ活性显著低于１６ｗｋ以前．结论自由基在实验性胃癌及其癌前病变发生中具有一定作用，自由基清除剂可能对胃癌的综合防治具有积极意义
Objective To explore the role of free radicals in the development of gastric cancer and its precancerous lesions. Methods One hundred Wistar rats were divided into two groups. The experimental group (70 rats) was treated with 100 mg/L aqueous solution of MNNG for 30 wk, and the control group (30 rats) was given drinking water. Select five timephases to dynamically observe malondialdehyde (MDA), lipid peroxidation (LPO), and glutathione peroxidase (GSH) in rat MNNG-induced experimental gastric cancer and its precancerous lesions. PX) and superoxide dismutase (SOD) changes. Results In the experimental group, the average content of MDA was significantly greater than 0wk (P<001) at 52wk, and significantly greater than 16wk before (P<005). The content of MDA in gastric cancer was significantly higher than that in precancerous lesions of gastric cancer (P<005). The content of LPO in cancer tissue was significantly higher than that in precancerous lesions (P<005). In the experimental group, the activities of total SOD and CuZnSOD were significantly lower than 16 weeks before 52wk (P<005 and P<001, respectively). The content of CuZnSOD in cancer tissue was significantly lower than that in normal gastric mucosa (P<001), and was also significantly lower than that in gastric mucosa dysplasia and intestinal metaplasia (P<005). The activities of GSH-PX at 30 wk and 52 wk were significantly lower than 16 wk. Conclusion Free radicals play a role in the development of experimental gastric cancer and precancerous lesions. Free radical scavenger may have positive significance for the comprehensive prevention of gastric cancer.