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[Objective] To optimize the extraction conditions for the ultrasonic extraction of alkaloids from NIGHTSHADE.[Method] Based on single factor test,the extraction time,extraction temperature and solid-liquid ratio were selected as the independent variables.With the extraction rate of alkaloids as the response value,central composite design was adopted to research on the effects of independent variables and their overlapping effects on the extraction rate of alkaloids.Predictive function of quadratic regression equation was obtained by response surface method.And the optimal extraction condition for alkaloids from NIGHTSHADE was obtained.[Result] The optimal extraction condition was as follows:53 min extraction time,65 ℃ extraction temperature,1:20(g/ml) solid-liquid ratio,and 2 extraction times.Under this condition,the extraction rate of alkaloids from NIGHTSHADE reached 0.308%.[Conclusion] The optimal extraction condition for alkaloids from NIGHTSHADE was obtained,which provided references for the further development and utilization of NIGHTSHADE.
[Objective] To optimize the extraction conditions for the ultrasonic extraction of alkaloids from NIGHTSHADE. [Method] Based on single factor test, the extraction time, extraction temperature and solid-liquid ratio were selected as the independent variables .With the extraction rate of alkaloids as the response value, central composite design was adopted to research on the effects of independent variables and their overlapping effects on the extraction rate of alkaloids. Predictive function of quadratic regression equation was obtained by response surface method. And the optimal extraction condition for alkaloids from NIGHTSHADE was obtained. [Result] The optimal extraction condition was as follows: 53 min extraction time, 65 ° C extraction temperature, 1: 20 (g / ml) solid- liquid ratio, and 2 extraction times. of alkaloids from NIGHTSHADE reached 0.308%. [Conclusion] The optimal extraction condition for alkaloids was obtained from NIGHTSHADE was, which provided refer ences for the further development and utilization of NIGHTSHADE.