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研究地塞米松对脂多糖(LPS)介导的巨噬细胞和树突状细胞(DC)激活的影响。分离小鼠骨髓巨噬细胞分3组,即对照组、LPS组和地塞米松加LPS组。用rm-GM-CSF和rm-IL-4诱导小鼠骨髓来源的DC,分3组,即对照组、LPS组和地塞米松加LPS组。用ELISA法检测培养上清中的肿瘤坏死因子-α(TNF-α)和IL-12含量,用流式细胞仪检测CD40、CD86的表达。巨噬细胞LPS组TNF-α和DC LPS组TNF-α、IL-12、CD40、CD86表达增加,地塞米松可以显著降低上述表达水平。地塞米松对LPS激活巨噬细胞和DC有明显的抑制作用,并因此对LPS引起的炎症反应有保护作用。
To investigate the effect of dexamethasone on lipopolysaccharide (LPS) -mediated macrophage and dendritic cell (DC) activation. Mice bone marrow macrophages were separated into 3 groups: control group, LPS group and dexamethasone plus LPS group. Bone marrow-derived DCs were induced by rm-GM-CSF and rm-IL-4 in 3 groups: control group, LPS group and dexamethasone plus LPS group. The contents of tumor necrosis factor-α (TNF-α) and IL-12 in culture supernatants were detected by ELISA, and the expressions of CD40 and CD86 were detected by flow cytometry. The expression of TNF-α, IL-12, CD40 and CD86 in TNF-αand DC-LPS groups increased in macrophage LPS group. Dexamethasone could significantly reduce the above expression levels. Dexamethasone markedly inhibits LPS-activated macrophages and DCs and therefore has a protective effect on the inflammatory response induced by LPS.