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目的:了解婴幼儿人巨细胞病毒(HCMV)活动性感染的临床表现,以及与糖蛋白B(gB)基因型的关系。方法:ELISA法检测HCMV-IgM确定的HCMV阳性的婴幼儿51例,对其不同临床症状进行分析。对其中43例患儿使用套式PCR(nPCR)法加限制性长度多态性分析(RFLP)进行HCMV gB基因分型。结果:在51例HCMV感染患儿中全身性感染和单脏器感染分别占25.49%和74.51%,HCMV包涵体病和肝炎分别占25.49%和21.57%。43例患儿HCMV gB的基因分型结果为,gBI型20例,gB II型7例,gB III型9例,gB I、II混合型4例,gB I、III混合型2例,gB II、III混合型1例,未发现gB IV型。gB I型占46.51%。结论:婴幼儿HCMV感染临床表现种类多样;HCMV感染者以gB I型HCMV占多数。
Objective: To investigate the clinical manifestations of active infection of infantile human cytomegalovirus (HCMV) and its relationship with gB genotype. Methods: 51 cases of HCMV-positive infants and young children confirmed by HCMV-IgM were detected by ELISA, and their different clinical symptoms were analyzed. HCMV gB genotyping was performed on 43 of these children using nested PCR (nPCR) plus restriction-length polymorphism (RFLP). Results: In 51 children with HCMV infection, systemic infection and single organ infection accounted for 25.49% and 74.51% respectively. Incidence of HCMV inclusion body disease and hepatitis accounted for 25.49% and 21.57% respectively. The results of genotyping of HCMV gB in 43 children were as follows: gBI 20 cases, gB II 7 cases, gB III 9 cases, gB I, II mixed 4 cases, gB I, III mixed 2 cases, gB II , III mixed type in 1 case, no gB type IV was found. gB I accounted for 46.51%. Conclusion: There are many clinical manifestations of HCMV infection in infants and young children. HCMV infection accounts for the majority of HCMV gB type I infection.