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目的的体外研究选择性环氧合酶-2(COX-2)抑制剂塞来昔布和5-氟尿嘧啶(5-FU)对人胃癌BGC-823细胞增殖能力及其对VEGF和Survivin m RNA表达的影响。方法体外培养胃癌BGC-823细胞,MTT法检测塞来昔布在不同浓度下,不同时间对于BGC-823增殖的影响,并计算IC50值;PCR法检测塞来昔布联用5-FU对BGC-823细胞VEGF和Survivin m RNA表达的影响。结果MTT结果显示:相同干预浓度塞来昔布抑制BGC-823增殖,其24h,48h,72h的IC50分别为:(255.36±21.1)umo L/L、(149.12±2.29)umo L/L、(89.95±1.61)umo L/L。RT-PCR法检测结果显示,塞来昔布、5-FU均可抑制VEGF和Survivin m RNA的表达,且联合应用效果最强。结论塞来昔布可抑制人胃癌细胞的增殖,其于5-FU抗肿瘤机制可能通过抑制VEGF和Survivin的表达,从而对胃癌细胞起到协同抑制增殖的作用。
Objective To investigate the effect of COX-2 inhibitor celecoxib and 5-fluorouracil (5-FU) on the proliferation and the expression of VEGF and Survivin mRNA in human gastric cancer BGC-823 cells in vitro Impact. Methods BGC-823 cells were cultured in vitro. The effect of celecoxib on the proliferation of BGC-823 cells was detected by MTT assay at different times and the IC50 values were calculated. The effect of celecoxib combined with 5-FU on BGC-823 -823 cells VEGF and Survivin m RNA expression. Results The results of MTT showed that the IC50 of 24h, 48h and 72h were (255.36 ± 21.1) umol L / L, (149.12 ± 2.29) umol L / L, ( 89.95 ± 1.61) umo L / L. The results of RT-PCR showed that celecoxib and 5-FU could inhibit the expression of VEGF and Survivin m RNA, and the combination effect was the strongest. Conclusion Celecoxib can inhibit the proliferation of human gastric cancer cells. The anti-tumor mechanism of 5-FU may inhibit the proliferation of gastric cancer cells by inhibiting the expression of VEGF and Survivin.