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甘薯,旋花科,一年生植物,我国近年来种植面积达1亿亩,占世界的80%以上,成为最大的甘薯生产国.甘薯是一种杂种优势作物,但采用营养繁殖导致病毒病蔓延,致使产量和质量降低.甘薯品种退化的诸因素中病毒占主要.病毒病已成为我国甘薯生产的最大障碍之一,每年造成损失50亿元以上.侵染甘薯的病毒病有十多种,我国甘薯上发生的病毒主要有甘薯羽状斑驳病毒(SPEMV)和甘薯潜隐病毒(SPLV)病毒在无性繁殖体中逐代积累,造成质量和产量下降.而甘薯茎尖生长点生长速度快,0.3mm～0.5mm长的带1～2个叶原基的茎失不带病毒,因此取甘薯顶部及腋芽的尖端进行组织培养,可脱去病毒.
Sweet potato, Convolvulaceae, annual plant, China’s acreage in recent years reached 100000000 acres, accounting for more than 80% of the world, becoming the largest sweet potato producing country.Fruit sweet potato is a heterosis crop, but the use of vegetative propagation led to the spread of the virus disease, Resulting in reduced yield and quality of the sweet potato variety degradation of the virus among the main factors.Viral disease has become one of the biggest obstacles to the production of sweet potato in China each year, causing more than 5 billion yuan loss.Water infecting sweet potato virus disease there are more than 10 kinds of our country The viruses that occur on sweet potato are mainly sweet potato feather mottle virus (SPEMV) and sweet potato latent virus (SPLV) virus accumulate one by one in vegetative propagules, resulting in the decrease of quality and yield, while the growth of sweet potato shoot tip is fast, 0.3 mm ~ 0.5mm long with 1 or 2 leaf primordia stems without virus, so take sweet potato tops and tips of axillary bud tissue culture, can take off the virus.