In this paper, by using concentration and carbon stable isotope the.CO2 sources of soil profiles developed on limestone, dolostone and claystone basements in Central Guizhou, China are comparatively studied. The results show that CO2 concentration of soil profiles developed on different basements is different, having the following sequence: limestone>dolostone>claystone. Below the soil depth of 20 cm from the surface the δ13C value of CO2 in soil profile developed on limestone ranges from -12.811‰ - -13.492‰(PDB), that in soil profile developed on dolostone varys from -13.212‰--14.271‰(PDB) and that in soil profile developed on claystone is about -20.234‰- -21.485‰(PDB). Taking the carbon isotope of soil organic matter and carbonate rock as two isotopic endmembers, the proportion of soil C02 generated by dissolution of carbonate rock is calculated, about 21%-25% for soil profile developed on limestone basement, 19%-21% for soil profile developed on dolostone basement. There is almost no influx of In this paper, by using concentration and carbon stable isotope the.CO2 sources of soil profiles developed on limestone, dolostone and claystone basements in Central Guizhou, China are comparatively studied. The results show that CO2 concentration of soil profiles developed on different basements is different , having the following sequence: limestone> dolostone> claystone. Below the soil depth of 20 cm from the surface the δ13C value of CO2 in soil profile developed on limestone ranges from -12.811 ‰ - -13.492 ‰ (PDB), that in soil profile developed on dolostone varys from -13.212 ‰ - 14.271 ‰ (PDB) and that in soil profile developed on claystone is about -20.234 ‰ - 21.485 ‰ (PDB). Taking the carbon isotope of soil organic matter and carbonate rock as two isotopic endmembers, the proportion of soil C02 generated by dissolution of carbonate rock is calculated, about 21% -25% for soil profile developed on limestone basement, 19% -21% for soil profile developed on dolostone basement. Ther e is almost no influx of