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目的探讨过氧化物酶增殖物激活受体γ(PPARγ)、环氧合酶2(COX-2)在宫颈癌中的表达与HPV感染的关系。方法采用免疫组化法检测PPARγ、COX-2在宫颈癌和CIN中的表达,原位杂交法检测HPV16DAN。结果(1)PPARγ和COX-2在对照组、CIN和宫颈癌组中的阳性表达率分别为0、55.56%、88.37%和0、75.39%、83.72%,各组间阳性表达差异有统计学意义(P<0.05)。(2)HPV16在正常对照组、CIN和宫颈癌组中的阳性表达率分别为5%、46.3%、46.51%,对照组与各组间阳性表达差异有统计学意义(P<0.01),CIN与宫颈癌组间阳性表达率差异无统计学意义(P>0.05)。(3)宫颈癌组织PPARγ和COX-2的表达有协同作用(r=0.322,P=0.035)。结论宫颈癌中PPARγ、COX-2的表达并不因HPV16的感染而消失,其转录功能仍然存在;PPARγ和COX-2在CIN和宫颈癌中异常表达,提示其与肿瘤的发生密切相关,检测PPARγ、COX-2有助于宫颈癌早期诊断。
Objective To investigate the relationship between the expression of peroxisome proliferator - activated receptor γ (PPARγ) and cyclooxygenase 2 (COX - 2) in cervical cancer and HPV infection. Methods Immunohistochemistry was used to detect the expression of PPARγ and COX-2 in cervical cancer and CIN. HPV16DAN was detected by in situ hybridization. Results (1) The positive rates of PPARγ and COX-2 in control group, CIN and cervical cancer group were 0, 55.56%, 88.37% and 0,75.39%, 83.72% Significance (P <0.05). (2) The positive expression rates of HPV16 in normal control group, CIN and cervical cancer group were 5%, 46.3% and 46.51%, respectively. There was significant difference between positive control group and control group (P <0.01) There was no significant difference between the two groups (P> 0.05). (3) The expression of PPARγ and COX-2 in cervical cancer tissues had a synergistic effect (r = 0.322, P = 0.035). Conclusions The expression of PPARγ and COX-2 in cervical cancer does not disappear due to the infection of HPV16, but transcriptional function still exists. The abnormal expression of PPARγ and COX-2 in CIN and cervical cancer suggests that it is closely related to the tumorigenesis. PPARγ, COX-2 contribute to the early diagnosis of cervical cancer.