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为了研究乙型肝炎病毒（HBV）前S1／S2基因变异规律及其与HBV感染临床转归的关系，我们选择了三组不同转归的HBV感染者：①急性乙型肝炎患者（完全恢复）；②慢性HHV携带者；③慢性重型乙型肝炎患者。每组各3例。用半巢式PCR法从此9例HBV感染者血清中扩增出前S1／S2片段，并且?
In order to study the variation of pre-S1 / S2 gene variation of hepatitis B virus (HBV) and its relationship with the clinical outcome of HBV infection, we selected three different groups of patients with HBV infection: ① patients with acute hepatitis B (complete recovery) ; ② chronic HHV carriers; ③ chronic severe hepatitis B patients. 3 cases in each group. Pre-S1 / S2 fragments were amplified from the serum of 9 HBV infected patients by semi-nested PCR.