目的 :证实人牙乳头细胞中 BMP- 7的表达 ,获得 h BMP- 7成熟肽基因。方法 :提取人牙乳头细胞总 RNA,反转录合成 c DNA,以特异性引物扩增 h BMP- 7成熟肽基因并克隆入 T载体 ,筛选阳性克隆进行序列测定。结果 :PCR扩增得到一特异性的约 430 bp的片段 ,克隆后筛选出阳性克隆 ,序列测定结果表明与国外已发表的序列完全一致。结论 :人牙乳头细胞中有 BMP- 7基因的表达 ,并克隆得到 h BMP- 7成熟肽基因 ,为 h BMP- 7在牙齿发育中的作用及其功能研究奠定了基础 Objective: To confirm BMP-7 expression in human dental papilla cells and to obtain h BMP-7 mature peptide gene. Methods: Total RNA was extracted from human dental papilla cells and reverse transcribed into c DNA. The h BMP-7 mature peptide gene was amplified by specific primers and cloned into T vector. The positive clones were screened and sequenced. Results: A specific fragment of about 430 bp was amplified by PCR. After cloning, the positive clones were screened out. The results of sequencing showed that they were identical with the published sequences in foreign countries. CONCLUSION: The expression of BMP-7 gene in human papillary cells and the cloning of h BMP-7 mature peptide gene lay the foundation for the study of the function and function of h BMP-7 in tooth development