In the past dozen years,inductively coupled plasma mass spectrometry(ICP-MS)has emerged as a simple and spectacular tool for quantitative bioanalysis [1-5].We focused on the study of quantitative bioanalysis of proteins [6-10],viruses [11] and cells [12] using ICP-MS along with development of the chemoselective and biospecific lanthanide(Ln)-tagging strategies in the past several years.A designed and synthesized chemical hub that orthogonally integrates elemental and molecular mass spectrometry enables us to know what biomolecules have been quantified by ICP-MS [13].Now,we are developing metabolism-based and bioorthogonal click chemistry mediated Ln-tagging strategies for counting the D-Alanine that crosslinks in vivo in the peptidoglycan-based bacterial cell wall,and the glycoproteins overexpressed on cell surface.Such methodological developments will benefit deeper understanding of the intrinsic roles of biomolecules in a certain biological pathway.We are on the way to approach such a goal.