【摘 要】
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With the completion of human genome sequencing, one of the challenges of proteomics is to separate and identify protein encoded by genomes with high resolution, high efficiency, high sensitivity and h
【机 构】
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Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dali
【出 处】
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DaLian Internationall Symposia and Exhibition on Chromatogra
论文部分内容阅读
With the completion of human genome sequencing, one of the challenges of proteomics is to separate and identify protein encoded by genomes with high resolution, high efficiency, high sensitivity and high throughput.Under most situations, without enzymatic digestion, proteins could hardly be unambiguously identified by MS because of the relative low ionization efficiency.Therefore, proteins should be degraded into fragments to obtain the sequence information.The most common protocol is accomplished by free solution digestion, which generally takes at least several hours.Therefore, it is indispensable to speed up such a procedure.
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