不同浓度腐胺对人肝细胞增殖、凋亡的影响

来源 :第九届全国创伤修复(愈合)与组织再生学术会议暨第二届中欧创面修复学术会议 | 被引量 : 0次 | 上传用户:wusyun
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
  目的:探讨不同浓度腐胺对体外培养人正常肝细胞增殖、凋亡的影响.方法:将体外培养的人正常肝细胞株LO2随机分为实验组与对照组,实验组培养液中加入腐胺,并设置10个浓度,分别为0.25、0.5、1、10、20、40、80、160、320、640μg/mL,对照组不添加腐胺.
其他文献
目的:研究受体活性修饰蛋白1(receptor activity modifying protein 1,RAMP1)过表达对MG-63细胞降钙素受体样受体(calcitonin receptor-like receptor, CRLR)表达和分布的影响,探讨RAMP1过表达后对降钙素基因相关肽(calcitonin gene-related peptide,CGRP)促进MG-63细胞分化中作用
背景:我们前期研究发现,组蛋白去乙酰化酶2 (HDAC2)具有独立的SUMO E3连接酶功能结构域,通过此酶介导,HDAC2可通过上调翻译起始因子eIF4E的SUMO化修饰,直接参与并促进蛋白质翻译起始复合物eIF4F的形成,上调cap-依赖蛋白质翻译水平。目的:构建HDAC2的SUMO E3连接酶功能失活的片段缺失突变体,探究此HDAC2新功能是否影响小鼠威纤维细胞L929的增殖。
Keratinocytes proliferation is critical for the capacity to heal wounds and accumulating evidences have proved that dysregulation of microRNAs is involved in proliferation of keratinocytes.However, th
Objective: Fetuses and adults follow different repair strategies for the healing of skin wounds.Skin wound healing is primarily controlled by the proliferation and migration of dermal fibroblasts as w
Islet cell replacement therapy represents a promising approach for the cure of type 1 diabetes if autoimmunity to β cells is under control.However, this potential is limited by a shortage of pancreas
目的:观察壳聚糖蜂蜡膏对大鼠创面中CD34、羟脯氨酸的影响及创面愈合效果并探讨其可能机制。方法:取20只雄性SD大鼠,在其背部两侧制造全层皮肤缺损创面,制成创面模型,每只大鼠背部三个创面随机分为实验组、对照组和空白对照组。造模后每隔2d换药1次,空白对照组不做处理,实验组创面使用5g壳聚糖蜂蜡膏,对照组使用5ml生理盐水。
Objective: Adipose tissue derived stem cell (ADSCs) transplantation has shown great promises for enhancing skin repair in animal models of diabetic skin wound healing.However, poor viability of transp
Objectives: CD34+ peripheral hematopoietic stem cells have surrogated the marrow for almost all autologous and the majority of allogeneic hematologic and nonhematologic diseases.Efficient methods for
目的:研究Maspin在结肠癌中的表达情况,探讨Maspin与结肠癌临床病理因素和预后的关系。方法:采用免疫组织化学方法检测45例结肠癌,23例结肠腺癌和19例癌旁正常组织中Maspin蛋白的表达,并对其与结肠癌临床病理分期及预后的关系进行统计学分析。
Objective: Fetal wound healing occurs rapidly and without scar formation early in gestation, but the mechanisms underlying this scarless healing are poorly understood.This study explores the phenotypi