Traditionally,the characterization of nerve agents(NAs) and the evaluation of the effectiveness of antidotes are studied by enzyme assay of acetylcholinesterase(AChE) [1],which has several limitations duo to lack of specificity and easy to be interfered by other AChE inhibitors.In our research,according to the analytical toxicology strategies [2],a series of HPLC-MS/MS-SRM methods for quantatively determining effective biomarkers of NAs exposure have been developed to explain the processes of AChE poisoning,reactivation and aging when it is attacked by NAs or treated by reactivators.