【摘 要】
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Objective To detect the alterations of the telomerase activity and the expression for genes response to oxidative stress related with cellular senescence during the replicative senescence and hydrogen
【机 构】
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School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515
【出 处】
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中国毒理学会生化与分子毒理专业委员会学术会议暨环境内分泌干扰物的毒理学研究新技术新方法研讨会
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Objective To detect the alterations of the telomerase activity and the expression for genes response to oxidative stress related with cellular senescence during the replicative senescence and hydrogen peroxide-induced premature senescence in human embryonic lung fibroblasts (HEFs) in vitro.Methods The HEFs were divided into young cells (22 population doubling levels, 22PDL), mid-aged cells (35PDL) and replicative senescent cells (49PDL) and premature senescent cells induced by H2O2 (the premature senescence persistence group, PSp) according to the age definition in cell culture.The telomerase activity was detected by ELISA assay during cellular replicative and premature senescence.The rnRNA level of genes response to oxidative stress was performed by fluorescence quantitative PCR for Foxol, Foxo3, apoA-I and PDX1 separately related with cellular senescence.Results The telomerase activity was not detected in young cells, and it increased in mid-aged cells and replicative senescence stages obviously during cellular replicative senescence.The telomerase activity in premature senescence was also highly active.The mRNA level for Foxol, Foxo3, apoA-I and PDX1 was decreased separately with increased passages in normal HEFs during cellular replicative senescence.In premature senescence, the mRNA level was only decreased for Foxol, Foxo3 and apoA-I, but increased for PDX1 significantly.Conclusion The telomerase has no activity in young cells, going up with increased PDLs.Senescent cells have high telomerase activity, regardless of replicative or premature.The expressions for genes related with cellular senescence have differences between replicative and premature senescence.
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