The identification of a sensitive and specific serum marker will facilitate the early diagnosis ofhepatocellular carcinoma (HCC) and increase patient survival rates.In this study, bile fluid from 82 patients suffering from hepatic carcinoma (n=40), gall-stone (n=20), cystic polypi (n=15) and hepatic benign lesions (n=7) were analyzed through a high-efficient Ultra Performance Liquid Chromatography (UPLC) mass spectrometry metabolic profiling strategy.The acquired data were subjected to orthogonal signal correction-partial least squares discriminate analysis (OSC-PLSDA) to extract the significant bile fluid components that played a key role in defining the disease state.