Detection of trace target compounds in plant samples is difficult because of the complex matrix.In phytohormon analysis, the sample preparation method is a key step regarding accuracy, reproducibility and detection limit of overall analysis.Phytohormon such as gibberellins (GAs) have to be extracted and isolated from plant samples before analysis.The traditional extraction techniques used for GAs, such as liquid-liquid extraction (LLE) and solid-phase extraction (SPE), have drawbacks of multi-step, time-consuming, require more than 20 mL solvents.