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The aim of this study was to evaluate the effect of NT-3 to decrease the differentiation time of bulge stem cells of rat hair follicle to neuron like cells.The bulge region of the rat whisker was isolated and cultured in DMEM/F12 supplemented with epidermal growth factor (EGF) in 3 groups for 7,8 and 9 days.Then 10 ng/ml NT-3 were added to each group for 3 days.Finally cell differentiation was assessed by Immunocytochemistry usingβ Ⅲ-tubulin antibody and the result compared with control groups.