Combination of metformin and curcumin exhibits synergistic inhibitory effects on tumor growth and me

来源 :中国药理学会第十四次全国学术大会暨施维雅优秀青年药理学工作者奖20周年学术论坛 | 被引量 : 0次 | 上传用户:gxwy1305
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  OBJECTIVE Hepatocellular carcinoma (HCC) is the most common cause of cancer-related mortality, with high incidence rates, robust metastatic propensity and acquired resistance to therapy.
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目的 借助斑马鱼胚胎建立神经胶质瘤模型以研究神经胶质瘤体内增值、迁移的分子机制,可以高效的进行抗肿瘤药物的筛选.方法 首先使用细胞生物学方法建立荧光标记的胶质瘤细胞系,去内毒素红色荧光质粒pcDNA3.0+DsRed的扩增及其线性化处理,去内毒素质粒小提试剂盒提取pcDNA3.0+DsRed质粒.使用显微注射法将胶质瘤细胞移植入斑马鱼体内,最后使用原位杂交、荧光定量RT-PCR、血管内源性碱性磷酸
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