Angiogenesis is involved in the development,progression and metastasis of various human cancers.Herein,we report the discovery of glipizide,a widely used drug for type 2 diabetes mellitus,as a promising anticancer agent through the inhibition of tumor angiogenesis.By high-throughput screening (HTS) of an FDA approved drug library utilizing our in vivo chick embryo chorioallantoic membrane (CAM) and yolk sac membrane (YSM) models,glipizide has been identified to significantly inhibit blood vessel formation and development.Moreover,glipizide was found to suppress tumor angiogenesis,tumor growth and metastasis using xenograft tumor and MMTV-PyMT transgenic mouse models.