Development of the Fc-Ⅲ Tagged Protein Expression System and its Application for Studying Protein Co

来源 :第八届中国蛋白质组学大会 | 被引量 : 0次 | 上传用户:zhuxin99
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Protein tagging involves genetically grafting a peptide sequence onto a recombinant protein.These fusion proteins have variousapplications in molecular biology such as affinity purification,protein detection,and epitope presentation.Recently we havedeveloped the Fc-Ⅲ tagged protein expression and purification system.
其他文献
Chronic stressful events are key risk factors for major depressive disorder (MDD),yet some individuals exposed to stressful eventsdo not develop MDD.This disparity suggests the significance of resilie
Objective To investigate the changes of proteomics in serum of Sprague-Dawley(SD)rats after accumulated irradiation by 137Cs γ ray.Methods Thirty mature SD rats were randomly divided into three groups
Glyco-site identification (glycoproteomics) and glycan elucidation (glycomics) represent two divided directions for protein glycosylation analysis,whereas site specific glycan structures become more i
Background:Colon cancer is the third leading cause of cancer death and there have been a growing trend In recent years,affecting over a million people annually worldwide.However most people are diagno
Isobaric chemical tags (e.g.TMT,iTRAQ) are powerful tools for quantitative proteomics,which are based on the release of lowmass reporter ions during fragmentation,and enable concurrent identification
Drug resistance poses a major challenge to ovarian cancer treatment.Understanding mechanisms of drug resistance is importantfor finding new therapeutic targets.
Chemical cross-linking combined with mass spectrometry (CXMS) is a powerful tool for analyses of protein structures and proteinprotein interactions.We have developed a CXMS workflow,which combines a v
会议
The Zoige wetland of the Tibetan plateau is at permanent low temperatures and is a methane emission heartland of the plateau;Strain R15,a novel psychrophilic methanogen,was isolated from the methanol
Ovarian cancer is the major cause of death in women with gynecological cancer because of a lack of early diagnosis markersand fast progression.A deep understanding of ovarian cancer biology and findin
At first,we optimized separation protocol of membrane proteins of Aspergillus fumigates by 2D Fluorescence difference gelelectrophoresis (2D DIGE).We found that membrane proteins were dissolved well i