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In order to study the effects of Trichoderma asperellum ACCC30536 on the nutrient components of soil, pot culturing experiment of 1-year-old tissue-cultured Populus davidiana x P.bolleana seedlings was carried out.Both soil in empty pots and soil in seedling-culturing pots were treated with T.asperellum conidia solution at the concentration of 5 x 103 cfu · cm-3 soil (as T and T1), where soil in negative and soil in a seedling-culturing pot without treatment were taken as control groups (Con and Con1, respectively).