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To establish a human ovarian carcinoma cell line with directional highly lymphatic me-tastasis and study of their biological characteristics.Through the methods and the results , consider that the ovarian carcinoma sub-line with directional highly lymphatic metastasis potential was established successfully. The ovarian carcinoma with directional highly lymphatic metastasis is a complex process involving multiple factors and genetic changes .The cells could provide a good experimental material for further investigation on lymphatic metastasis of ovanan carcinoma.