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＜正＞Tumor-oriented nanocarrier drug delivery approaches with pH-sensitivity have been drawing considerable attentions over the years.Here we describeda liposomal delivery system modified withpH-responsive cell penetrating peptide TH （TH-Lip）.Conventional cell penetrating peptide（CPP）-related drug delivery tactics sometimes seemed limited due to the extensive in vivo penetratbn and the lack of proper selectivity of conventional CPPs.In this study,TH （AGYLLGHINLHHLAHL（Aib）HHIL-NH2）,an engineered a-helical cell penetrating peptide originated from peptide TK（AGYLLGKINLKKLAKL（Aib）LLIL-NH2）,was endowed pH-responsiveness after complete replacement of all lysines in the sequence of TK into hisdines,and was introduced onto the surface of liposomes.Accordingly, TH-Lip could benefit from the unique property of TH,as the cell penetrating capacity of TH was concealed during the blood circulation and in normal tissues because of the neutral pH under those conditions.However,when TH-Lip reached the tumor,and as pH declined,hisitidines in TH peptide protonated and the surface charge of TH-Lip converted from negative to positive,initiating activated cell penetrating capacity and leading toenhanced cellular and tumor spheroid uptake.The endocytosis inhibition assay demonstrated that the endocytosis of TH-Lip was influenced by the positively charged surface of the liposomes in acidic environment and was mediated by clathrin, and the intracellular trafficking study suggested that the hposomes were mainly accumulated in endoplasmic reticulum and Golgi apparatus.After systemic administration in mice,TH-Lip could be internalized into tumor cells efficaciously. When it comes to the delivery of paclitaxel（PTX）,the pH-responsiveness of TH-Lip led to strong inhibition against tumor cell growth which occurred both in vitro（under pH 6.3） and in vivo,and the tumor inhibition rate reached 86.3%on C26 tumor-bearing mice for PTX-loaded TH-Lip.Therefore,TH-Lip proved itself to be a promising pH-responsive strategy for drug delivery within acidified tumor microenvironment.